Evaluation of the hepato-renal functions and antimicrobial activity of fatty amido benzoic acid synthesised from Citrullus colocynthis seed oil

Abstract Rapid progression in resistance to antimicrobial agents by pathogenic organisms is a serious concern. This study aimed synthesize fatty amido benzoic acid (FBA) from Citrullus colocynthis seed oil (CCO) and evaluate its safety profile as an alternative bioactive agent for combating drug-resistant pathogens. FBA was synthesised through simple chemical reaction route examined antioxidant activity capacity against selected microorganisms. Effect of on hepato-renal function makers oxidative stress also using Wistar rats. Density functional theory (DFT) approach employed understand the action with aid lowest unoccupied molecular orbital (LUMO) highest occupied (HOMO). Gas Chromatography (GC) revealed most abundant CCO be C18:2 (55.88%). Results Fourier transformed infrared spectroscopy (FTIR), proton nuclear magnetic resonance ( 1 HNMR) confirmed synthesis yield 97.10%. exhibited potential (IC 50 1.96 µg mL −1 ) well potency. Minimum inhibitory concentration (MIC) 0.026 mg . Biochemical parameters such alanine aminotransferase (ALT), aspartate (AST), creatinine, hydrogen peroxide, lipid peroxidation were significantly elevated rats administered high dose (100 kg ). Histology liver kidney biochemical results. Furthermore, mechanism could described quantum analysis via nucleophilic interaction, which may viewed electronically donor–acceptor interaction. The presents promising organisms.